Optical traps for single molecule biophysics: a primer
نویسنده
چکیده
Optical trapping experiments of different complexities are making a significant impact in biology. This review seeks to highlight design choices for scientists entering the field or building new instruments and to discuss making calibrated measurements with optical traps. For specificity, this review focuses on nucleic acid-based assays, but the discussion reflects the general experimental design considerations of developing a biological assay and an optical trap to measure it. An optically trapped bead is used to mechanically stretch a single DNA molecule. Optical traps for single molecule biophysics: a primer
منابع مشابه
Development of Single - Molecule Force and Torque Measurement with Application to Nucleosome Disruption
Title of Document: DEVELOPMENT OF SINGLE-MOLECULE FORCE AND TORQUE MEASUREMENT WITH APPLICATION TO NUCLEOSOME DISRUPTION. Jen-Chien Chang, Doctor of Philosophy, 2014 Directed By: Assistant Professor Arthur La Porta, Department of Physics Single-molecule force spectroscopy is a powerful method in biophysical research. The ability of detecting and manipulating single molecule finds applications i...
متن کاملForce spectroscopy with dual-trap optical tweezers: molecular stiffness measurements and coupled fluctuations analysis.
Dual-trap optical tweezers are often used in high-resolution measurements in single-molecule biophysics. Such measurements can be hindered by the presence of extraneous noise sources, the most prominent of which is the coupling of fluctuations along different spatial directions, which may affect any optical tweezers setup. In this article, we analyze, both from the theoretical and the experimen...
متن کاملÅngström-precision optical traps and applications.
Single-molecule optical-trapping experiments are now resolving the smallest units of motion in biology, including 1-base-pair steps along DNA. This review initially concentrates on the experimental problems with achieving 1-Å instrumental stability and the technical advances necessary to overcome these issues. Instrumental advances are complemented by insights in optical-trapping geometry and s...
متن کاملOptothermal biophysics: Light driven molecule traps, Microscale Thermophoresis, Imaging of kinetics and Autonomous evolution
Light driven molecule traps. Trapping single ions under vacuum allows for precise spectroscopy in atomic physics. However the confinement of biological molecules in bulk water is hindered by the lack of comparably strong forces. Molecules have been immobilized to surfaces, often with detrimental effects on their function. Here, we optically trap molecules by creating the microscale analog of a ...
متن کاملHigh-resolution, single-molecule measurements of biomolecular motion.
Many biologically important macromolecules undergo motions that are essential to their function. Biophysical techniques can now resolve the motions of single molecules down to the nanometer scale or even below, providing new insights into the mechanisms that drive molecular movements. This review outlines the principal approaches that have been used for high-resolution measurements of single-mo...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2009